Figure 1 from P4HA1 Mediates Hypoxia-Induced Invasion in Human Pancreatic Cancer Organoids
Year: 2025
Type: other
Abstract: Hypoxia increases invasion in patient-derived PDAC organoids. A, Schematic of PDAC organoid culture generation, collection, study, and analysis timeline. B, Effect of hypoxia on invasion in PDO1, PDO4, and PDO11 organoids. Percent invasion represents a mean of minimum three biological replicates with SD. Each biological replicate n > 30 organoids (P = 0.015, P = 0.0032, and P = 0.1636, respectively, t test). C, Fold change of PDAC PDO sample invasion in normoxia and hypoxia. D, Representative images of PDO4 invasive organoids in normoxia and hypoxia. Scale bars, 100 μm. E, Inverse circularity scores of normoxic and hypoxic PDO1 (n = 15 and n = 29, respectively; P = 0.0122, t test), PDO4 (both n = 71; P = 0.0025, t test), and PDO11 (n = 44 and n = 54, respectively; P = 0.0024, t test) invasive organoids. F, Expression level of EMT markers (E-cadherin, N-cadherin, vimentin, and SNAIL) in PDO4 measured by qRT-PCR at multiple time points. Fold changes, expressed relative to normoxic control samples, represent a mean of three technical replicates with SD. G, Western blot analysis of EMT markers (fibronectin, E-cadherin, N-cadherin, and vimentin) in PDO4 in normoxia and hypoxia. H, hypoxia; N, normoxia.
Authors: Bernat Navarro-Serer, Maria F. Wissler, Brandi K. Glover, Michael G. Lerner, Harsh H. Oza, Vania Wang, Hidur Knutsdottir, Fatemeh Shojaeian, Kathleen Noller, Saravana Gowtham Baskaran, Sarah Hughes, Alana M. Weaver, Daniel Wilentz, Oluwatobiloba Olayemi, Joel S. Bader, Elana J. Fertig, Daniele M. Gilkes, Laura D. Wood
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410901264