Figure S4 from The Prolonged Half-Life of the p53 Missense Variant R248Q Promotes Accumulation and Heterotetramer Formation with Wild-Type p53 to Exert the Dominant-Negative Effect
Year: 2025
Type: other
Abstract: Figure S4A illustrating that heterotetramerization is required for the DNE in vivo when xenografting MOLM13-TP53 WT cells overexpressing either tetramerization-proficient (R248Q) and tetramerization-deficient (R248Q-L344P) in NSG mice and treated with Idasanutlin. Figure S4B showing FACS data of how electroporation of plasmids encoding WT p53 into cells with endogenous TP53R248Q (K562-TP53R248Q-p21-GFP) almost fully restored the p21-GFP reporter signal.
Authors: Nancy Klemm, Roman R. Schimmer, Nils K. Konrad, Flavian Thelen, Jonas Fullin, Ebru Topçu, Christian Koch, Milena Treacy, Matthew Joseph Leventhal, Marco M. Bühler, Veronika Lysenko, Alexandre P.A. Theocharides, Kari J. Kurppa, Stefan Balabanov, Tuncay Baubec, Andrei V. Krivtsov, Peter G. Miller, Scott A. Armstrong, Benjamin L. Ebert, Markus G. Manz, Cesar Nombela-Arrieta, Steffen Boettcher
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410971199