Work

Methods for the study of membrane physiology, synaptics, immunohistochemistry, morphology and ultrastructure of intrinsic cardiac neurons from humans, mice, and pigs v1

Year: 2024

Type: preprint

Abstract: Intrinsic cardiac neurons (ICNs) play a crucial role in the proper functioning of the heart; yet a paucity of data pertaining to human ICNs exists. We took a multidisciplinary approach to complete a detailed cellular comparison of the structure and function of ICNs from mice, pigs, and humans. Immunohistochemistry of whole and sectioned ganglia, transmission electron microscopy, intracellular microelectrode recording and dye filling for quantitative morphometry were used to define the neurophysiology, histochemistry, and ultrastructure of these cells across species. Here described are the extended methodology for these experiments to accompany the published manuscript and dataset. https://doi.org/10.26275/6hdr-oe0p

DOI: 10.17504/protocols.io.3byl4998zgo5/v1

Authors: John D Tompkins, Donald Hoover, Leif Havton

Institution: University of California, Los Angeles

Language: English

Topic: Heart Rate Variability and Autonomic Control

Subfield: Cardiology and Cardiovascular Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Scheme for Promotion of Academic and Research Collaboration, National Institutes of Health, Common Fund

Awards: Transmission Electron Microscopy Service for SPARC Consortium Investigators, G4217170409, Comprehensive Structural and Functional Mapping of the Mammalian Cardiac Nervous System