Supplemental Figure 3 from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target
Year: 2025
Type: supplementary-materials
Abstract: Supplemental Figure 3: Anti-CD19 control MAbs HIB19 and CB19 were screened on a CD19 alanine-scan mutation library expressed in HEK 293T cells, with binding assayed by flow cytometry. Binding values are shown as a percentage of the antibodies’ binding to the parental CD19 protein (WT) and represent the mean of at least two replicate data points, with error bars showing half of the range (half of the maximum minus minimum value). The mutations highlighted are ones in which binding of 42 scFv was significantly impaired.
Authors: Divanshu Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel, Shannon Christensen, Ting-Jia Fan, Decheng Song, Yanping Luo, Yanling Wang, Huaishan Wang, Regina M. Young, Carl H. June, John Scholler, James L. Riley
Language: English
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4411039861