Work

Unveiling the Potential Binding Targets of Celastrol in Colorectal Cancer: A Proteomic Profiling Approach Integrating Cellular Thermal Shift Assay and Pulse Proteolysis

Year: 2025

Type: article

Abstract: Celastrol, a natural compound classified as a pentacyclic triterpenoid, has demonstrated efficacy in inhibiting human colorectal cancer cells' growth, adhesion, and metastasis through various signaling pathways. However, the specific protein target responsible for the effects of celastrol remains unclear, limiting its potential for further applications in colon cancer treatment and drug development. In this study, we propose a novel approach by combining a cellular thermal shift assay and pulse proteolysis techniques to identify the potential binding proteins of celastrol. Utilizing proteomic profiling on 2-dimensional electrophoresis, we successfully identified eight potential binding targets. MALDI-TOF mass spectrometry was conducted to verify these proteins as EEF2, STIP1, GAPDH, FLNA, SETSIP, GANAB, TXNDC17, and PRDX2. Our results provide valuable insights into the protein targets through which celastrol exerts its pharmacological effects, opening up new avenues for targeted therapies and drug development in colorectal cancer.

Source: Journal of Proteome Research

DOI: 10.1021/acs.jproteome.4c00738

Authors: Ti Lin, Shang-Lin Yang, Chao-Jung Chen, Pei-Fen Liu

Institutions: National Chung Hsing University, National Taiwan University, National Cheng Kung University, China Medical University, China Medical University

Language: English

Topic: Natural Compounds in Disease Treatment

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500