Year: 2025
Type: article
Abstract: Werner helicase-interacting protein 1 (WRNIP1) is a member of the AAA+ ATPase family and is conserved from Escherichia coli to Homo sapiens. Although a role for WRNIP1 in the S phase of the cell cycle has previously been reported, the present study demonstrates a novel function for this protein in G1 phase. Deletion of WRNIP1 in non-homologous end joining (NHEJ)-deficient chicken DT40 cell lines resulted in slow growth, accumulation in G1 phase, an increased population of dead cells, and accumulation of double-strand breaks (DSBs), which were indirectly evaluated by γH2AX. The data suggest that WRNIP1 prevents the generation of DSBs, executes DSB repair, and/or regulates DSB repair independently of NHEJ, particularly in G1 phase. The potential molecular mechanism by which WRNIP1 functions in G1 phase is discussed.
Authors: Akari Yoshimura, Takuya Abe, Masayuki Seki
Institution: Tohoku Medical and Pharmaceutical University
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4411081457