Work

Structural characterization and immunomodulatory activity of a polysaccharide produced by chaetomium globosum CGMCC 6882

Year: 2025

Type: article

Abstract: Efficient utilization of cellulose resources is of great significance to environmental protection and to solve the crisis of global energy shortage. In the present work, a polysaccharide (CGP-TS) was successfully produced by Chaetomium globosum CGMCC 6882 from waste tobacco stalk via submerged fermentation. Chemical composition analysis showed that the carbohydrate and protein contents in CGP-TS were 92.55% ± 3.16% and 4.73% ± 1.04%, respectively. Structural characterization indicated that CGP-TS was not pectin and composed of rhamnose, arabinose, galactose, glucose and xylose in a molar ratio of 22.2166: 9.8269: 36.2455: 3.3111: 0.416, and its molecular weight was 613.235 kDa. In vitro bioactivity and potential application investigation showed that antioxidant activities of CGP-TS against DPPH, ABTS, hydroxyl and superoxide radicals were positively correlated to its concentration from 0.5 mg/mL to 2.5 mg/mL. Meanwhile, CGP-TS exhibited immunomodulatory activity via enhancing the phagocytic activity and promoting the cytokines (TNF-α, IL-1β, IL-6 and NO) release of RAW 264.7 macrophages.

Source: Scientific Reports

DOI: 10.1038/s41598-025-05693-8