Embryonic cerebrospinal fluid pressure in fetal mice in utero : External factors pressurize the intraventricular space
Year: 2025
Type: article
Abstract: Background Previous experiments inducing leakage of embryonic cerebrospinal fluid (CSF) suggest the necessity of intraventricular CSF pressure ( P CSF ) for brain morphogenesis. Nevertheless, how embryonic P CSF occurs is unclear, especially in utero . Results Using a Landis water manometer, we measured P CSF in fetal mice isolated from the amniotic cavity ( P CSF‐ISO ) and found that P CSF‐ISO rose from 20 Pa at embryonic day (E) 10 to 100–110 Pa at E14–16. At E13, intraventricular injections of ≥3 μL of saline elevated P CSF‐ISO by ~30%, whereas those of inhibitors of CSF secretion decreased P CSF‐ISO by ~30%. Shh‐mediated cerebral wall expansion did not significantly increase P CSF‐ISO . Removal of the brain‐surrounding contractile tissues decreased P CSF‐ISO by 80%–90%. We then found that the intraamniotic pressure measured in utero ( P AF‐IU ) declined from 2000 Pa at E10 to 500 Pa at E15–18 but was always much greater than P CSF‐ISO . Direct measurement of P CSF in utero ( P CSF‐IU ) at E13 and E15 coupled with the measurement of P CSF‐ISO under hydrostatic pressure loading to mimic P AF‐IU at various embryonic ages revealed the following relationship: P CSF‐IU = P CSF‐ISO + P AF‐IU . Conclusions The P CSF of mice in utero is influenced by external factors, most strongly by intraamniotic pressure and less strongly by brain‐confining tissues.
Source: Developmental Dynamics
DOI: 10.1002/dvdy.70047
Institution: Nagoya University
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,430
OpenAlex ID: W4411093719