Year: 2025
Type: article
Abstract: Unspecific peroxygenases (UPOs) are promising biocatalysts for selective oxyfunctionalization. Compared to cytochrome P450 enzymes (P450s), the catalytic potential of UPOs has been less investigated, largely due to their limited natural diversity and the challenges associated with their optimization through enzyme engineering. In this study, we engineered an UPO from Aspergillus niger (AniUPO) to catalyze the enantioselective α-hydroxylation of β-ketoesters, a valuable transformation yet to be realized in biocatalysis. Through enzyme engineering, two AniUPO variants, AniUPO-M3 and AniUPO-M6, were developed to produce a wide range of enantioenriched α-hydroxy-β-ketoesters, achieving up to 97% yield, 4140 total turnover number (TTN), and >99:1 enantiomeric ratio (er). The biocatalytic process operates under mild conditions and is scalable for preparative applications. This study broadens the catalytic repertoire of UPOs and enhances their potential for industrial applications.
Authors: Zhen‐Yu Wang, Xin‐Yuan Ma, Ying Wu, Yang Liu, Guang‐Xin Lin, Xiao‐Qi Liu, Chun Zhang, Peng Chen, Yongxiang Zheng, Zhi‐Jun Jia
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Award: G3546029467
OpenAlex ID: W4411100150