Figure 3 from Targeting the Glucose–Insulin Link in Head and Neck Squamous Cell Carcinoma Induces Cytotoxic Oxidative Stress and Inhibits Cancer Growth
Year: 2025
Type: other
Abstract: GLUT1 inhibition enhances cytotoxic oxidative stress in human HNSCC cell lines. A,In vitro proliferation and immunoblot analyses of shScramble (shScr) and shRNA-mediated KD of GLUT1 (shGLUT1) in HNSCC cell line FaDu (n = 4 for each group). Two-way ANOVA. ****, P < 0.0001 (left). Immunoblot validation of shRNA-mediated KD of GLUT1 in FaDu cells (right). B, Relative glucose uptake and (C) intracellular ROS measurement in shScr and shGLUT1 FaDu cells (n = 3 for each group). Two-tailed t test. ***, P < 0.001; **, P < 0.01. D,In vitro proliferation of shScr, shGLUT1, and shGLUT1 + NAC (5 mmol/L) in HNSCC cell line SCC-61 (n = 4 for each group). Two-way ANOVA. ****, P < 0.0001 (left). Immunoblot validation shRNA-mediated KD of GLUT1 in SCC-61 cells (right). E, Intracellular ROS measurement of shScr, shGLUT1, and shGLUT1 + NAC (5 mmol/L) in SCC-61 cells (n = 3 for each group). Two-tailed t test. ****, P < 0.0001; ***, P < 0.001. F and G,In vitro proliferation of FaDu cells treated with GLUT1 inhibitors, WZB117 (0–40 μmol/L) or BAY-876 (0–100 nmol/L; n = 3 for each group). H and I,In vitro proliferation of FaDu (H) or SCC-61 (I) treated with WZB117 (30 μmol/L) or BAY-876 (150 nmol/L) with or without 5 mmol/L NAC treatment (n = 6 for each group). Two-way ANOVA. ****, P < 0.0001; **, P < 0.01; *, P < 0.05 (top). Intracellular H2O2 measurement of FaDu (H) or SCC-61 (I) cells treated with WZB117 (30 μmol/L) or BAY-876 (150 nmol/L) with or without 5 mmol/L NAC treatment (n = 6 for each group). Two-tailed t test. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01 (bottom). All error bars represent mean ± SEM unless otherwise noted. BAY, BAY-876; RFU, relative fluorescent unit; RLU, relative light unit; WZB, WZB117.
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4411103160