Work

Integrated physiological, metabolomic, and transcriptomic analyses highlight the involvement of JA regulatory pathway in the induced resin biosynthesis of masson pine

Year: 2025

Type: article

Abstract: Masson pine ( Pinus massoniana Lamb.) is a crucial resin-producing tree species in China. Enhancing induced resin biosynthesis is a vital method to augment resin yield. To elucidate the mechanisms underlying induced resin biosynthesis in masson pine, currently, the stems were subjected to wounding and MeJA treatment for physiological, metabolomic, and transcriptomic analyses, so as to uncover the key genes and regulatory pathways involved in induced resin biosynthesis. The results demonstrated that exogenous MeJA significantly induced the formation of traumatic resin ducts. Both wounding and exogenous MeJA remarkably increased terpene synthase activities and JAs contents. Transcriptome analysis revealed that key genes, e.g. LOX , AOS , OPR , etc., involved in JA biosynthesis were highly up-regulated after wounding and MeJA treatment. JA signaling was activated, and the key transcription factors (JAZs, MYCs, etc.) involved in this pathway were differentially expressed. The expression of the function genes participated in terpenoid biosynthesis ( TPSs , CYP720Bs , HDRs , etc.) was diversely activated. PmHDR2 exhibited high expression in masson pine stems, and its expression level was significantly positively correlated with resin yield. PmHDR2 -overexpressed transgenic tobacco effectively improved its chlorophyll content, plant growth, as well as monoterpenes and sesquiterpenes contents, accordingly promoted highly terpenoids accumulation, further reflecting the regulatory function of JA in resin biosynthesis. Therefore, wounding and exogenous MeJA substantially activated the JA signal transduction pathway, subsequently up-regulating terpenoid biosynthesis-related genes, leading to increased terpenoid biosynthesis. These results provide a theoretical implication for improving resin yield via genetic improvement and plantation technology.

Source: Industrial Crops and Products

DOI: 10.1016/j.indcrop.2025.121331

Authors: Zijing Zhou, Zhengchun Li, Fuhua Fan, Luonan Shen, Xiaopeng Wen

Institutions: Guizhou University, Zunyi Vocational and Technical College

Language: English

Topic: Plant biochemistry and biosynthesis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,720

Funders: Guizhou University, Science and Technology Program of Guizhou Province