The Influence and Potential Mechanism of Acute Myocardial Infarction on Bone Metabolism in Pigs
Year: 2025
Type: article
Abstract: 目的：本研究旨在探究急性心肌梗死(AMI)对猪骨代谢的影响及潜在机制。方法：本研究用12头8周龄雄性约克夏猪作为实验动物，随机分为手术组(n = 6)及假手术组(n = 6)。手术组猪使用左前降支动脉结扎法建立心肌梗死模型，假手术组进行相同的手术，但未结扎左前降支。(1) 测量并比较两组猪的基线数据；(2) 两组猪术前及术后30 d LVEF的检测、白细胞分析、血清钙、磷及PTH水平的及骨代谢指标检测；(3) 双能X射线吸收法测定两组实验猪术后30 d腰椎(L1~L4)及左股骨骨密度。(4) 对两组猪于术后30 d取右股骨进行H&E染色，显微镜下观察并比较两组实验猪骨小梁面积。结果：1) 两组猪基线数据比较均无统计学差异(P > 0.05)。2) 手术前，两组LVEF值无显著差异(P > 0.05)；手术后，与假手术组相比较，手术组的LVEF值显著下降(P P > 0.05)。② 手术前两组单核细胞计数无显著差异(P > 0.05)；手术后30 d手术组单核细胞计数显著升高(P P > 0.05)；与假手术组相比，术后30 d手术组血钙、血磷水平显著下降(P P > 0.05)；手术组在术后30 d的甲状旁腺素水平显著高于假手术组(P P > 0.05)；手术组在术后30 d的25-(OH)D、骨钙素N端中分子片段水平显著低于假手术组(P P Objective: The purpose of this study is to investigate the effects of acute myocardial infarction (AMI) on bone metabolism in pigs and its potential mechanisms. Methods: This study used 12 8-week-old male Yorkshire pigs as experimental animals, divided into surgical group (n = 6) and sham surgery group (n = 6). The surgical group of pigs was subjected to a left anterior descending artery ligation method to a myocardial infarction model, and the sham surgery group underwent the same surgery but did not ligate the left anterior descending branch. (1) The baseline data in the two groups of pigs were measured and compared; (2) The detection of LVEF of the two groups of pigs before and after 30 days of surgery, white blood cell analysis, levels of serum, phosphorus and PTH, and detection of bone metabolism indicators; (3) The bone mineral density of the lum spine (L1~L4) and left femur of the two groups of experimental pigs was measured by dual-energy X-ray absorptiometry 30 days surgery. (4) The right femur of the two groups of pigs was taken for H&E staining on postoperative day 30, and the trabecular of the two groups of experimental pigs was observed and compared under the microscope. Results: 1) There was no statistically significant difference in the baseline data size of the two groups of (P > 0.05). 2) There was no significant difference in the LVEF value size of the two groups before surgery (P > 0.05); the LVEF value size of the surgical group was significantly lower than that of the sham surgery group after surgery (P P > 0.05). ② Preoperatively, monocyte counts showed no significant difference between groups (P > 0.05); the monocyte count of the two groups was significantly higher after 30 days of surgery and was statistically significant (P P > 0.05); the blood calcium and blood phosphorus size of the surgical group was significantly lower than that the sham surgery group after surgery (P P > 0.05); the parathyroid hormone levels of the surgical group were significantly higher than those of the sham surgery at 30 days after surgery (P P > 0.05); the levels of 25-(OH)D and N-terminal mid-molecular fragment of osteocalcin in the surgical group were significantly lower than of the sham surgery group at 30 days after surgery (P P < 0.05). 7) Under the microscope, the trabecular area of the right femur in the surgical group was smaller than that in the sham surgery group in a single field of. Conclusion: This study shows that myocardial infarction can lead to an increase in the overall bone resorption markers in pigs, resulting in a decrease in bone mass and bone density.
Source: Advances in Clinical Medicine
Author: 楠楠 冷
Language: English
Subfield: Orthopedics and Sports Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4411158437