An Improved m6A-ELISA for Quantifying N6-methyladenosine in Poly(A)-purified mRNAs
Year: 2025
Type: article
Abstract: -methyladenosine quantification, including mRNA isolation, can be achieved in two days. • Describes a robust method for poly(A) RNA isolation from total RNA, minimizing non-poly(A) RNA contamination. • Based on Ensinck et al. [1], optimizing poly(A) RNA selection from yeast and mammalian cells and reducing the amount of mammalian mRNA for the assay.
Source: BIO-PROTOCOL
Authors: Wei Chan, Waleed Albihlal, Folkert Van Werven
Institution: The Francis Crick Institute
Language: English
Topic: RNA modifications and cancer
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4411161658