Exploration of the mechanism of a novel enhancer on buffalo muscle development
Year: 2025
Type: article
Abstract: The developmental process of skeletal muscle involves a wide variety of regulatory factors. However, little has been reported regarding the function and mechanisms of enhancer regulation in buffalo skeletal muscle growth and development. In this study, we discovered an enhancer that affects the buffalo skeletal muscle growth and development, which we named Enh67. Experimental results from CCK-8, EdU, qPCR, and Western blot assays indicated that Enh67 inhibits the proliferation of buffalo muscle stem cells. Immunofluorescence staining, qPCR, and Western blot results showed that Enh67 promotes the differentiation of buffalo muscle stem cells. Finally, we determined through 3C and qPCR assays that Enh67 targets CEP85L. Our findings not only enrich the regulatory factors involved in buffalo skeletal muscle growth and development but also provide potential target candidates for improving meat production and treating muscle development-related diseases in livestock.
Source: Genomics
Authors: Saixing Duan, Xingyu Liu, Wenjing Liu, Yuting Dou, Gaoxiao Xu, Feng Zhang, Kuan Geng, Qi Xiong, Mengjie Chen
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,980
Award: G2833594544
OpenAlex ID: W4411196453