Work

Effect of fluoride toothpaste and mouthwash on the cytotoxicity of buccal mucosa cells in the presence of nickel-titanium archwires during comprehensive orthodontic treatment: A randomized clinical trial

Year: 2025

Type: article

Abstract: Objective To evaluate the effect of fluoride on the cytotoxicity of buccal mucosa cells in the presence of nickel-titanium archwires. Materials and methods Seventy-five subjects requiring comprehensive orthodontic treatment were randomly allocated to three groups. Healthy control group included normal subjects who did not receive orthodontic treatment. In the treatment control group, only stainless-steel attachments were placed without archwire. Experimental group included subjects in whom stainless-steel attachments and 0.014″ nickel-titanium archwires were used. Fluoride mouthwash and toothpaste were prescribed to all subjects. Buccal mucosa cell smear was assessed for cell viability, micronucleus scores and caspase-3 reactive cells just before (T 0 ) and 3-months (T 1 ) after the intervention. Results The buccal mucosa cell viability score was significantly lower in the experimental and treatment control groups than in the healthy control group subjects (P < 0.001). Micronucleus score was significantly higher in the experimental and treatment control groups compared to the healthy control group subjects (P < 0.001). The caspase-3 reactive cells and micronucleus scores were significantly more in the experimental group subjects compared to treatment control group subjects (P < 0.001). There was a negative but statistically non-significant correlation between cell viability and micronucleus score among three groups of subjects. Conclusion The use of fluoride mouthwash and toothpaste in the presence of stainless-steel attachments and nickel-titanium archwire decreased the buccal cell viability by 8.51 % and increased the micronucleus score by 3.03 %. The use of fluoride toothpaste and mouthwash had a significant cytotoxicity effect on buccal mucosa cells in the presence of nickel-titanium archwire during comprehensive orthodontic treatment.

Source: Journal of Oral Biology and Craniofacial Research

DOI: 10.1016/j.jobcr.2025.06.007

Authors: Mayur Bhola, Ashok Kumar Jena, Madhusmita Sethy, Jitendra Sharan

Institutions: All India Institute of Medical Sciences Bhubaneswar, All India Institute of Medical Sciences

Language: English

Topic: Orthodontics and Dentofacial Orthopedics

Subfield: Orthodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,800