Work

Controlled liquid-liquid phase separation via the simulation-guided, targeted engineering of the RNA-binding protein PARCL

Year: 2025

Type: article

Abstract: experiments to validate these predictions. To investigate the RNA-binding of PARCL, we added microRNA to the simulations and identified a short region of PARCL that consistently made contact with the miRNA in agreement with bioinformatics predictions and experiments. We discuss the implications of our findings in terms of model-guided engineering of biomolecular condensates.

Source: iScience

DOI: 10.1016/j.isci.2025.112852

Authors: Ruth Veevers, Steffen Ostendorp, Anna Ostendorp, Julia Kehr, Richard J. Morris

Institutions: John Innes Centre, Universität Hamburg

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,240

Funders: Deutsche Forschungsgemeinschaft, Horizon 2020 Framework Programme, European Research Council, H2020 European Research Council