Work

Discrepancies in qPCR-based gene quantification and their dependencies on soil properties, inhibitor presence, and DNA extraction kit types

Year: 2025

Type: article

Abstract: ion spiking experiments were performed to observe multiple inhibitory effects on qPCR analysis performance. The results suggest that discrepancies in gene quantification are evident in the presence of qPCR inhibitors in soil samples. Furthermore, discrepancies in quantification results are exacerbated by gDNA template quality, which is attributed to DNA extraction. The observed multiple inhibitory effects underscore the importance of careful consideration of both DNA template quality and soil type to ensure more accurate gene quantification in soils.

Source: RSC Advances

DOI: 10.1039/d5ra02689j

Authors: Eunhye Lee, Hyun Jeong Lim, Ahjeong Son

Institution: Ewha Womans University

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,042

Funders: National Research Foundation, National Research Foundation of Korea