Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study
Year: 2025
Type: article
Abstract: Background: The therapeutic potential of herbs in treating various diseases is gaining recognition due to their promising outcomes and minimal side effects. One such herb, Nigella sativa , is renowned for its medicinal properties, particularly due to its extract, Thymoquinone (TQ), which demonstrates anti-inflammatory, anti-oxidant, antimicrobial, analgesic, and immune-modulating effects. Green synthesis methods for nano-sized particles are considered environmentally friendly and have been proven effective given their size, higher surface-to-volume ratio, and enhanced physical and mechanical properties. This study aims to investigate a novel approach in the green synthesis of silver nanoparticles of Nigella sativa and 0.1% TQ and to assess their anti-inflammatory and antioxidant activities . Materials and Methods: The seeds of NS were used to green synthesize silver nanoparticles. The anti-inflammatory effects of the green synthesized particles were tested using egg albumin and bovine serum assays, and their anti-oxidant actions were assessed using 2,2-Diphenyl-1-picrylhydrazyl denaturation assay and H 2 O 2 assay. Results: The novel formulation of Nigella sativa -mediated silver nanoparticles with 0.1% TQ exhibits 76% anti-inflammatory activity and 80%–87% anti-oxidant properties. Conclusion: Our results suggest that the silver nanoparticles mediated from N. sativa with 0.1% TQ extract could serve as an effective herbal adjunct for treating periodontal infections because of its potent anti-inflammatory and anti-oxidant properties.
Authors: Leslie Rani Sthanislas, Bagavad Gita Jayaraman, Gautham Kumar Nandhakumar, C. S. Krishnan, Gowri Shankar Nattuthurai, Menaka Vasudevan, Savithri Nediyaparambu Krishnakumar, Noblin Shyma
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4411216734