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In Vitro Study of the Effects of Postbiotic Mouthwash Prototype with Cannabis Extracts on the Reduction of Inflammatory Cytokine: Tumor Necrosis Factor Alpha

Year: 2025

Type: article

Abstract: Objective: This study evaluated the effects of postbiotic mouthwash containing cannabis extracts (CBD) on the reduction of tumor necrosis factor alpha (TNF-α) levels in vitro. Material and Methods: Seven mouthwash formulations were prepared using CBD at concentrations ranging from 0.25% to 1.0% v/v. TNF-α reduction was assessed using a lipopolysaccharide (LPS) model to activate the THP-1 human monocytic cell line (ATCC, TIB202). The enzyme-linked immunosorbent assay (ELISA) technique was employed to quantify TNF-α.Results: All formulations reduced TNF-α levels compared to the control. The most effective formulations (Preparations 1–4) achieved approximately 91% inhibition. Formulations 5–7 showed inhibition levels of 49.3%, 80.9%, and 82.5%, respectively.Conclusion: The postbiotic mouthwash containing CBD effectively reduced TNF-α levels, indicating its potential for clinical application in inflammation management.

Source: Journal of Health Science and Medical Research

DOI: 10.31584/jhsmr.20251225

Authors: Nattapon Nisapar, Malai Taweechotipatr, Malai Taweechotipatr, Sorasun Rungsiyanont

Institution: Srinakharinwirot University

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4411216869

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