Ixora coccinea flower extracts as a rapid test assay to confirm the proper pasteurization of milk
Year: 2025
Type: article
Abstract: Pasteurization is crucial for hygienic safety and milk shelf-life extension.Alkaline phosphatase (ALP) activity determines pasteurization efficiency, but most methods are time-consuming or require sophisticated instrument facilities, which are rare in rural India dairy or collection centers.The study developed a simple, quick, and economical chromogenic test protocol based on the activity of Alkaline Phosphatase (ALP) in raw milk.The dye was prepared from Ixora coccinea water extract.Pasteurization inactivates ALP, resulting in a brownish green colour.The assay has a sensitivity of >0.5 units/L, allowing for easy visualization of pasteurized milk compared to raw milk.
Source: Indian Journal of Animal Health
Authors: P. A. Mangroliya, T. Hazra, R. Sindhav, A. Solanki, R. Govani
Institutions: Parul University, Kamdhenu University
Language: English
Subfield: Biotechnology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4411221596