Work

Optimization of a real-time PCR assay for hepatitis B virus load determination in infected patients.

Year: 2025

Type: article

Abstract: Hepatitis B virus (HBV) infection is a significant health problem in the world, with around 294 million chronic carriers. Determination of viral load is crucial for the monitoring and treatment follow-up of HBV-infected pa-tients. In-house methods are economical alternatives for those settings where HBV viral load determination might not be widely available commercially. Mo-lecular diagnostic techniques need to take into account the variability of this virus. The aim of this study was the evaluation and optimization of a real-time PCR method for the determination of HBV load. After optimization, the in-house assay evaluated in this study showed acceptable sensitivity and specific-ity, allowing its use for monitoring patients in low-income settings.

Source: Investigación Clínica

DOI: 10.54817/ic.v66n2a04

Authors: María Zulay Sulbarán, Yoneira Fabiola Sulbarán, Carmen Luisa Loureiro, Héctor Rafael Rangel, Rossana Celeste Jaspe, Flor Helene Pujol

Institution: Instituto Venezolano de Investigaciones Científicas

Language: English

Topic: Hepatitis B Virus Studies

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Ministerio de Ciencia, Tecnología e Innovación Productiva