Work

Cloning-Free Targeting of Endogenous Loci to Generate Fluorescent Reporters in Medaka

Year: 2025

Type: article

Abstract: ). The method is simple, robust, and efficient, thus facilitating straightforward organismal genome editing. Key features • Cloning free CRISPR/Cas9 tagging of endogenous genes with fluorescent reporter sequences. • Guidelines for designing CRISPR/Cas9 endogenous tagging experiments. • Straightforward generation of transgenic Medaka knock-in reporter lines. • Versatility with the use of Cas9 mRNA or protein and various fluorescent reporters.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5360

Authors: Simon Knoblich, Kiyoshi Naruse, Ali Seleit, Alexandre Paix

Institutions: Heidelberg University, European Molecular Biology Laboratory, National Institute for Basic Biology, Max Planck Institute for Biology

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W4411249398

FWCI:

Cites:

Cited by:

Related to: 10