Work

Abstract 2587 Recombinant expression and purification of A. vinelandii CowN in E. coli

Year: 2025
Type: conference-abstract
Abstract: The goal of this project is to study the protein CowN in the diazotroph Azotobacter vinelandii (Av) in comparison to CowN in Gluconacetobacter diazotrophicus (Gd). Nitrogenase is an enzyme that catalyzes the reduction of nitrogen gas into ammonia in diazotrophic bacteria. In many diazotrophs, the protein CowN prevents nitrogenase inhibition from the environmental gas carbon monoxide (CO). Previous work studied CowN in the diazotroph G. diazotrophicus. In G. diazotrophicus, CowN binds to nitrogenase and weakens CO binding to its active site.
Authors: Julie Takei, Cedric Owens, Katie Sanders, Neeraja Gajendran
Language: English
Field: Energy
Open Access status: gold
Estimated APC paid: $3,430
OpenAlex ID: W4411273868
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Related to: 10