Work

Outside Back Cover: A Single‐Molecule Liposome Assay for Membrane Permeabilisation (Angew. Chem. Int. Ed. 26/2025)

Year: 2025

Type: article

Abstract: A single-molecule liposome assay for membrane permeabilization using confocal microscopy paired with a fast-flow microfluidics device is reported by Scott L. Cockroft, Mathew H. Horrocks et al. in their Research Article (e202503678). Liposomes, encapsulating calcium-sensitive dye, pass through the channel aligned with the microscope. Membrane-permeabilizing agents allow calcium influx, sensitizing the dye which is detected as the liposome passes through the confocal volume. As successive liposomes pass through the volume, signals acquired indicate the permeabilising properties of the analyte.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.202512274

Authors: Krzysztof M. Bąk, Daniel C. Edwards, Dylan George, Bhanu Singh, Ryan Ferguson, Tianxiao Zhao, Kristin Piché, Ariel Louwrier, Scott L. Cockroft, Mathew H. Horrocks

Institution: University of Edinburgh

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W4411293076

FWCI:

Cites:

Cited by:

Related to: 10