Year: 2025
Type: article
Abstract: Cells respond to environmental stresses such as cold and osmotic stresses. These stresses induce signal transduction pathways in cells. However, the molecular mechanisms activated by cold and osmotic stresses in higher eukaryotes remain elusive. Previously, we described a reporter system utilizing inducible translocation trap that detects nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase (KBL) in response to cold and osmotic stresses. In the present study, we developed additional reporter systems to detect intracellular events induced by these stresses. These reporter systems will be instrumental to elucidate the intracellular signaling mechanisms activated by these stresses.
Source: Biology Methods and Protocols
Authors: Kanon Maruyama, Hodaka Fujii
Institution: Hirosaki University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,120
OpenAlex ID: W4411299566