Galanin receptor agonists limit osteoclast number in in vitro mouse models
Year: 2025
Type: article
Abstract: Despite evidence that galanin-like compounds are potential regulators of bone activity, little research has been undertaken to investigate the role of these compounds on osseous cells. The aim of this study was to elucidate the mechanisms by which the galanin receptor agonists galanin and galnon regulate osteoclasts. Osteoclasts were cultured from femurs of C57BL/6 mice were grown to 80% confluence and differentiated via the application of mCSF and RANKL. Cells were analysed for osteoclast number via TRAP staining, qPCR and immunocytochemistry. A moderate dose of both galanin-compounds decreased osteoclast count. The moderate dose of galanin also reduced expression of inflammatory markers. These results indicate that both galanin and galnon can act to reduce osteoclast count, although they have led to contradictory responses with respect to inflammatory expression. These results may help to better treatment for bone-resorptive diseases such as osteoporosis.
Source: Fortune Journal of Rheumatology
Institution: La Trobe University
Language: English
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4411325263