Work

Integrated multi-omics and machine learning reveals immune-metabolic signatures in osteoarthritis: from bulk RNA-seq to single-cell resolution

Year: 2025

Type: article

Abstract: Purpose: The aim of this study was to investigate the activation of immune-metabolic pathways in osteoarthritis (OA) and their role in disease progression. We employed differential expression analysis and Gene Set Enrichment Analysis. Materials and methods: Gene set enrichment analysis (GSEA) to identify activated immune-metabolism pathways in OA. Subsequently, Weighted gene co-expression network analysis (WGCNA) was used to identify gene modules associated with OA and immune-metabolism scores, followed by enrichment analysis to reveal the functional characteristics of these modules. To identify immune-metabolism related differentially expressed genes (DEGs), we utilized seven machine learning methods, including lasso regression, random forest, bagging, gradient boosting machines (GBM), Xgboost-xgbLinear, Xgboost-xgbtree, and decision trees, to construct predictive models and validate their reliability. Based on the expression profiles of hub immune-metabolism related DEGs, we stratified OA patients into two immune-metabolism related subgroups and deeply investigated the differences in immune profiles, drug responses, functions, and pathways between these subgroups. Additionally, we analyzed the expression and pseudotime trajectories of hub immune-metabolism related DEGs at the single-cell level. Through genome-wide association studies (GWAS), we explored the mechanisms of action of hub immune-metabolism related DEGs. Finally, real-time polymerase chain reaction (RT-PCR) was utilized to verify the expression of hub immune-metabolism related DEGs. Results: Immune-metabolism related pathways were significantly activated during the development of OA. Thirteen central immune metabolism-related genes (CX3CR1, ADIPOQ, IL17RA, APOD, EGFR, SPP1, PLA2G2A, CXCL14, RARB, ADM, CX3CL1, TNFSF10, and MPO) were identified. Predictive modeling by constructing these genes has good predictive power for identifying OA. These genes are mainly associated with endothelial cells. Single-cell analysis showed that they were all expressed in single cells and varied with cell differentiation. RT-PCR results suggested that they were all significantly expressed in OA. Conclusion: Our findings indicate that immune metabolism plays a key role in the development of OA and provide new perspectives for future therapeutic strategies.

Source: Frontiers in Immunology

DOI: 10.3389/fimmu.2025.1599930

Authors: Hui He, Xiumei Zhao, Bo Zhang, Shijian Zhao, Yinteng Wu

Institutions: Guangxi Medical University, Youjiang Medical College for Nationalities, Kunming Medical University

Language: English

Topic: Osteoarthritis Treatment and Mechanisms

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801