Work

Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries

Year: 2025

Type: article

Abstract: This study utilized DNA-encoded library (DEL) screening to identify novel inhibitors of carbonic anhydrase 9 (CA9), a key cancer target. Among the identified compounds, CA9–005 exhibited strong inhibitory activity and reduced cancer cell viability while CA9–061 and CA9–062 showed high binding affinities but limited enzymatic inhibition. Biolayer interferometry confirmed CA9–005's direct interaction with CA9, supported by structural modeling. These findings highlight the potential of DEL screening for drug discovery and establish CA9–005 as a promising anticancer candidate, warranting further structural and therapeutic optimization.

Source: Results in Chemistry

DOI: 10.1016/j.rechem.2025.102455