Nitrite Causes Nitrosative Stress to Iron Sulfur Clusters
Year: 2025
Type: article
Abstract: Nitric oxide (NO), produced by nitrite reductases or nitric oxide synthases, performs vital roles in signaling and the immune response. Iron sulfur (FeS) clusters are known targets for NO induced degradation, serving as sensors to trigger cellular responses. However, this FeS reactivity is proposed as NO specific, with no demonstrated reactivity toward nitrite, a soluble NO storage molecule. We demonstrate that synthetic FeS clusters supported by various ligands undergo facile nitrosylation by nitrite in the presence of a reductant, evidencing the nitrite reductase reactivity for FeS clusters. Moreover, a mononitrosylated Fe 4 S 4 cluster, [tempS 3 Fe 4 S 4 (NO)] 2–, can be readily synthesized by this approach, enabling further investigation into the FeS cluster repair and decomposition under NO induced oxidative stress.
DOI: 10.1021/jacs.5c05636
Institutions: University of Florida, University of Nevada, Reno, Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Systèmes Moléculaires et nanoMatériaux pour l'Énergie et la Santé, Université Grenoble Alpes, Laboratoire de Chimie et Biologie des Métaux
Language: English
Field: Energy
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4411335692