Work

Phase-sensitive dynamic micro-optical coherence tomography for high-speed intracellular motion imaging

Year: 2025

Type: article

Abstract: Dynamic optical coherence tomography with micrometer resolution (DµOCT) offers enhanced contrast information by evaluating time-dependent signal fluctuations in images of living tissue. DµOCT's use has been limited to imaging excised fresh tissue specimens or 3D cell cultures due to the long observation times required, typically ranging from 1.35 to 25 seconds. To reduce the time needed to obtain DµOCT images, we developed a phase-based algorithm that analyzes intracellular motion by measuring phase changes between adjacent B-scans. This approach significantly reduces imaging time to as low as 40.5 ms while providing a quantitative measure of intracellular motion.

Source: Optics Letters

DOI: 10.1364/ol.563024

Authors: Hinnerk Schulz-Hildebrandt, Joseph A. Gardecki, Tiffany Miller, Maria Avila-Wallace, Erica Villareyna-Lopez, Guillermo Tearney

Institutions: Massachusetts General Hospital, Harvard–MIT Division of Health Sciences and Technology

Language: English

Topic: Optical Coherence Tomography Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: G4128959259, Dynamic µOCT for cellular tissue phenotyping