Year: 2025
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Infrared ion spectroscopy (IRIS) is a tandem mass spectrometry (MS) technique that generates structurally diagnostic vibrational spectra for mass-selected ions trapped in a mass spectrometer. Until now, IRIS applications for biological samples have primarily focused on solution-based analyses, such as body fluids (e.g., plasma and urine) and tissue homogenates, using electrospray ionization (ESI) coupled with liquid chromatography-mass spectrometry (LC-MS). In this study, we have combined matrix-assisted laser desorption/ionization (MALDI) with IRIS for the direct analysis of small molecules from biological tissues on a Fourier-transform ion cyclotron resonance mass spectrometer. We applied this technique alongside MALDI mass spectrometry imaging to analyse brain tissue from two knockout mouse models of l -lysine catabolism disorders: pyridoxine-dependent epilepsy ( ALDH7A1 ) and glutaric aciduria type 1 ( GCDH ). The MALDI-IRIS platform, now available for users at HFML-FELIX, represents a significant advance in the direct structural characterization of metabolites in complex biological tissues and opens new possibilities for structure elucidation in the field of MALDI mass spectrometry imaging.
Source: Analytical Chemistry
Authors: Jelle L. Schuurman, Lara van Tetering, Kas J. Houthuijs, Pieter Kooijman, Valerie Gailus-Durner, Stefanie Leuchtenberger, Helmut Fuchs, Martin Hrabe de Angelis, Udo F. H. Engelke, Dirk J. Lefeber, Clara D. M. van Karnebeek, Ron A. Wevers, Andrej Grgic, Benjamin Balluf, Michiel Vandenbosch, Rob Vreeken, Ron M. A. Heeren, Giel Berden, Jos Oomens, Jonathan Martens
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W4411348990