Work

Protocols to co-culture human primary lung cells in the simple-flow device

Year: 2025

Type: article

Abstract: Human lung models replicate various aspects to address diverse research questions. The complexity of human lung models, such as co-cultures and lung-on-chip devices, is increasing, but details on culture methodologies are often lacking. Here, we describe steps for the isolation, maintenance, and co-culturing of primary epithelial, endothelial, and mesenchymal cells derived from human lung resection material. We then detail procedures for 3D printing the simple-flow device, setting it up with co-cultures of human primary epithelial and endothelial cells under fluidic conditions.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103892

Authors: Cinta Iriondo, Sem Koornneef, Kari-Pekka Skarp, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Robbert J. Rottier

Institutions: Erasmus MC, Erasmus MC - Sophia Children’s Hospital

Language: English

Topic: 3D Printing in Biomedical Research

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550