Isolation and identification of Mycoplasma hyorhinis and virulence evaluation of its field isolates
Year: 2025
Type: article
Abstract: Introduction: , Mhr) is associated with various diseases, including multiple serositis, pneumonia, arthritis, and otitis media. It is also linked to the porcine respiratory disease complex (PRDC). Methods: prevalence in 2022 Chinese lung samples was assessed by species-specific PCR, followed by isolation and purification of field strains, followed by genetic characterization via multilocus sequence typing (MLST). Pathogenicity evaluation of three isolates (ZZ-1, GD-1 and AH-1) was evaluated using controlled piglet infection trials. Results: Mhr detection in clinical lung samples showed 31.77% prevalence. Three isolates (ZZ-1/ST166, GD-1/ST167, AH-1/ST144) were characterized by MLST. Piglet infection trials confirmed Mhr-induced polyserositis, pneumonia, and arthritis, with strain-dependent virulence variation observed. Discussion: as a high-prevalence pathogen (31.77%) in Chinese swine herds. Animal infection models demonstrated virulence variation among different Mhr strains. These findings contribute to identifying and assessing the threats posed by different strains to pig health, guiding the development of clinical prevention and control strategies.
Source: Frontiers in Veterinary Science
Authors: Fan Yang, Lijun Yang, Xuecheng Duan, Yulin Qian, Huifang Ma, Xue Jia, Xinyu Huo, Wenqi Dong, Huanchun Chen, Chen Tan
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
Awards: G1034635412, G3816309580
OpenAlex ID: W4411397221