Work

Unveiling the structure of Delftia acidovorans Lipopolysaccharide: insights into O-antigen and lipid A

Year: 2025

Type: article

Abstract: (max 250 words)Delftia acidovorans is a Gram-negative bacterium traditionally considered as an environmental organism, although it has recently been identified as an opportunistic pathogen in immunocompromised patients. Furthermore, this bacterium was also recognized as part of the crypt-specific core microbiota in healthy mice. Despite its detection in various contexts, the chemical structure of its lipopolysaccharide (LPS) remains uncharacterized.Here, the structure of LPS has been identified and both its components (O-antigen polysaccharide and Lipid A) have been characterized by combining chemical approaches with spectroscopic and spectrometric studies. In detail, the O-antigen is found to consist of a novel glycan sequence composed of 3)-α-L-rhamnose-(1→4)-α-D-glucose-(1→ disaccharide in the linear backbone, where glucose is in turn branched at the O-2 position with a β-D-N-acetyl-glucosamine, that is non-stoichiometrically acetylated at position O-6. Whereas lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution of acyl chains.To the best of our knowledge, this work is the first report describing in detail the structures of the O-antigen and lipid A within the Delftia genus.

Source: Carbohydrate Research

DOI: 10.1016/j.carres.2025.109586

Authors: Anna Notaro, Luisa Sturiale, Angelo Palmigiano, Domenico Garozzo, Immacolata Speciale

Institutions: University of Naples Federico II, Institute of Polymers, Composites and Biomaterials

Language: English

Topic: Carbohydrate Chemistry and Synthesis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,800