Work

Macrophage-targeted lipid nanoparticles based on Dendron-like lipids deliver mTOR inhibitor to alleviate systemic lupus erythematosus

Year: 2025

Type: article

Abstract: The dysregulation of the Mammalian Target of Rapamycin (mTOR) pathway in immune cells is strongly associated with disease activity and the development of lupus nephritis in patients diagnosed with Systemic Lupus Erythematosus (SLE). However, oral rapamycin demonstrates considerable inter-individual variability in bioavailability, and prolonged exposure to this agent may induce a wide range of adverse effects. Nanodelivery systems represent a promising strategy to optimize the therapeutic efficacy of rapamycin in the treatment of SLE. Herein, we develop an advanced strategy for SLE therapy utilizing lipid nanoparticles (LNPs) based on dendron-like lipids (DL-lipids) that deliver rapamycin to macrophages of spleen and bone marrow. Initially, a DL-lipid library featuring a variety of functional moieties was synthesized. And LNPs composed of the DL-lipids were formulated and screened for targeting immune organs and cells. Particularly, the LNP incorporating trifluorinated DL-lipid C18 3F is capable of accumulating in the spleen and bone marrow in a time-dependent manner and demonstrates macrophage affinity in vivo . Meantime, the rapamycin-loaded C18 3F LNP (Rapa@C18-3F) exhibits a synergistic effect that suppresses the activity of Mechanistic Target of Rapamycin Complex 1 (mTORC1) within macrophages, which in turn attenuates the progression of SLE by limiting immune activation, facilitating autophagosome degradation, and promoting macrophage polarization. Furthermore, the rapamycin resistance caused by repeated administration was reversed with the use of Rapa@C18-3F. Consequently, this approach presents a potential strategy for the treatment of SLE through the inhibition of the mTORC1 pathway by the administration of Rapa@C18-3F.

Source: Chemical Engineering Journal

DOI: 10.1016/j.cej.2025.165057