G-quadruplex-driven molecular disassembly and type I-to-type II photophysical conversion of a heavy-atom-free photosensitizer for site-specific oxidative damage
Year: 2025
Type: article
Abstract: selectively oxidizes guanine residues, triggering G4 unfolding, a mechanism validated through biophysical experiments, dot blot assay and molecular dynamics (MD) simulations. Furthermore, biochemical experiments at single-base resolution reveal that photoactivated DBI-POE induces site-specific oxidative lesions at G4 sites, stalling DNA polymerase, while non-G4 regions remain unaffected. This combination of supramolecular disassembly, photophysical pathway switching, and G4-selective oxidative damage underscores the high specificity of DBI-POE, opening new avenues for the design of next-generation G4-targeted PSs for photodynamic cancer therapies.
Source: Nanoscale Horizons
DOI: 10.1039/d5nh00237k
Institutions: Wrocław University of Science and Technology, Slovak Academy of Sciences, Institute of Molecular Biology of the Slovak Academy of Sciences, Centre National de la Recherche Scientifique, Institut des Sciences et Technologies Moléculaires d'Angers, Universidad Autónoma de Madrid, École Normale Supérieure de Lyon, Laboratoire de Chimie, Consejo Superior de Investigaciones Científicas, Instituto de Química Física Blas Cabrera
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: G1105844351, G1175115578, G121809054, Postdoctoral Fellowships in Poland to Boost International Mobility and Skills Training, G1855990336, G210041235, G2262748287, G2309174360, G2601397997, G2660406951, G279363373, G300979063, G3204934271, G34346518, G3480869486, G3681454997, G4042783231, G4178818211, G425668148, G430777504, G434337789, G4795296358, G491712853, G5080475149, G5195188938, G5404395142, G5421974903, G5476499552, G589680569, G5906552679, G624877701, Postdoctoral Fellowships in Poland to Boost International Mobility and Skills Training, G7084143925, G7258176174, G7398263626, G7805626310, G7863371703, G8497050386, G8624899980, G897484643, G99296377
OpenAlex ID: W4411414636