Work

Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice

Year: 2025

Type: article

Abstract: Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48 Cre/+ ; LSL-Kras G12D/+ (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facilitate the progression from acinar cells through acinar-to-ductal metaplasia (ADM) to pancreatic intraepithelial neoplasia (PanIN). We describe steps for AAV production, preparing KC mice, intra-pancreatic AAV injection, and analysis. This protocol allows the investigation of early events of pancreatic carcinogenesis in a spatially and temporally controlled manner. For complete details on the use and execution of this protocol, please refer to Li et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103907

Authors: Hongzhen Li, Zhao Shi, Shanshan Shen, Bo Kong

Institutions: Heidelberg University, Nanjing Drum Tower Hospital, Nanjing University, Nanjing Medical University

Language: English

Topic: Pancreatic and Hepatic Oncology Research

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550