Anti-obesity effects of Polygonum longisetum root extract via regulation of adipogenic pathways in differentiated 3T3-L1 cells
Year: 2025
Type: article
Abstract: Polygonum longisetum, commonly used in East Asian traditional medicine for its anti-inflammatory, antipyretic, and diuretic effects, has recently attracted attention for its antioxidant and anti-inflammatory activities.However, studies investigating the anti-obesity effects of P. longisetum root, particularly its molecular mechanisms related to adipocyte differentiation, remain limited.Therefore, this study aimed to evaluate the anti-obesity potential of P. longisetum root extract (PORE) using 3T3-L1 preadipocytes and to elucidate its effects on key transcription factors and signaling pathways involved in adipogenesis.PORE significantly reduced intracellular triglyceride accumulation during the differentiation of 3T3-L1 cells, as confirmed by Oil Red O staining, which showed a marked decrease in both lipid droplet size and number.Additionally, the extract effectively suppressed the expression of key adipogenic proteins, including Peroxisome proliferatoractivated receptor gamma, CCAAT/enhancer binding protein alpha, Sterol regulatory element-binding protein 1c, and Fatty acid-binding protein 4. Mechanistic analysis revealed that PORE inhibited the phosphorylation of mitogen-activated protein kinase (MAPK) family members (ERK1/2, p38, and JNK) and enhanced AMPK activation, suggesting its regulatory role in both MAPK and AMPK signaling pathways.These results indicate that P. longisetum root extract suppresses adipocyte differentiation and exerts anti-obesity effects by modulating energy metabolism and adipogenic signaling.This study provides scientific evidence supporting the potential use of P. longisetum root as a functional food ingredient and a natural anti-obesity agent, and suggests its value in the development of preventive and therapeutic strategies for obesity-related metabolic disorders. Keywords Adipogenesis • AMPK pathway • Anti-obesity • Mitogen-activated protein kinase signaling • Natural extract • Polygonum longisetum • 3T3-L1 cells 서 론 비만은 에너지 섭취와 소비의 불균형으로 인해 체내 지방조직이 과도하게 축적되는 상태로, 단순한 체중 증가를 넘어 다양한 대 사성 질환의 주요 위험 인자로 작용한다[1].특히, 지방세포의 분 화와 기능 이상은 인슐린 저항성, 염증 반응, 지질 대사 장애 등 과 밀접한 관련이 있다[2].지방세포 분화(adipogenesis)는 전지방세포(preadipocyte)
Authors: Jin Kyu Kang, Ye-Jin Lee, Chang-Gu Hyun
Institution: Jeju National University
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4411428670