Work

Effects of Perilla Seed Pomace Extract on Aging-Induced Cognitive Dysfunction in SAMP8 Mice

Year: 2025

Type: article

Abstract: Materials and Methods Perilla Seed Pomace Extract PreparationPSE, the residue remaining after oil extraction by pressing, was obtained from Kyungdong Oriental Market (Republic of Korea).To prepare the PSE, the perilla seed pomace was soaked in n-hexane to remove any residual Perilla seed pomace and its constituent phytochemicals have anti-inflammatory, antioxidant, and anti-aging benefits.However, its effects on aging-related cognitive decline are not well-studied.Therefore, the antioxidant and neuroprotective properties of perilla seed pomace extract (PSE) on hydrogen peroxide (H 2 O 2 )-induced neuronal cell death were examined.Additionally, the efficacy of PSE in ameliorating age-related cognitive impairment was evaluated in vivo using the senescenceaccelerated mouse prone 8 (SAMP8).PSE showed free radical scavenging activities and inhibited FeSO 4 -H 2 O 2 -induced lipid peroxidation.In HT22 neuronal cells, PSE protected against H 2 O 2 -induced oxidative stress and cytotoxicity by restoring mitochondrial membrane potential and attenuating excessive reactive oxygen species production.Furthermore, PSE reversed the H 2 O 2 -induced upregulation of apoptotic markers (Bax, cleaved caspase-3) and downregulation of the anti-apoptotic marker Bcl-2.Additionally, the protein levels of antioxidant enzymes (Catalase, SOD, and GPx) were increased under conditions of H2O2-induced oxidative stress.In vivo, PSE significantly ameliorated cognitive deficits, as evidenced by improved performance in passive avoidance and Morris water maze test results.Further, PSE reduced the accumulation of lipid peroxides and significantly increased catalase and glutathione peroxidase activity in the brain.These findings suggest that PSE attenuates agingrelated cognitive deficits by enhancing antioxidant defenses in the brain, highlighting its potential as a dietary intervention for aging-related cognitive decline.

Source: Journal of Microbiology and Biotechnology

DOI: 10.4014/jmb.2502.02007

Authors: Haeun Lee, Tae Youl Ha, Yun Tai Kim, Min Young Um

Institutions: Korea Food Research Institute, Korea University of Science and Technology

Language: English

Topic: Medicinal Plants and Neuroprotection

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Ministry of Science and ICT, South Korea, Korea Food Research Institute