Work

Artemisia argyi extracts alleviated colitis in mice via modulating gut microbiota and bile acid metabolism

Year: 2025

Type: article

Abstract: Artemisia argyi (A. argyi) is a Chinese herbal medicine with reported anti-inflammatory effects. In this study, the A. argyi was extracted with water and ethanol, and the concentrations of 35 flavonoids in A. argyi water extract (WE) and ethanol extract (EE) were measured via targeted metabolomics. The antioxidant and anti-inflammatory activities of both WE and EE were firstly explored in vitro via chemical assays and cellular experiment, respectively. Both WE and EE showed significant DPPH, ABTS, OH-, and O2- radical scavenging ability in a dose-dependent manner, and reduced the levels of IL-1β, TNF-α and IL-22 in LPS induced RAW264.7 cell model. In addition, the in vivo anti-colitis activity of both extracts was performed in dextran sulfate sodium (DSS)-induced colitis mouse, and the mechanisms were elucidated by 16S rDNA sequencing and targeted metabolomics. We found that both WE and EE relieved colitis in mice, characterized by decreased disease activity index, increased colon length, improved pathological changes in colon tissue, while EE showed better anti-colitis activity. In addition, both 16S rDNA sequencing and targeted bile acids metabolomics indicated EE modulated gut microbiota and specifically increased the abundance of lithocholic acid, which might contribute to intestinal barrier function improvement via up-regulating the expression of colonic FXR. In summary, this study identified the anti-colitis mechanism of A. argyi ethanol extract by modulating gut microbiota, facilitating the production of lithocholic acid, activating FXR and improving intestinal barrier function.

Source: Food Science and Human Wellness

DOI: 10.26599/fshw.2025.9250558