A pH and GSH dual responsive nanoparticle co-deliver Adriamycin and Olaparib enhanced the therapeutic efficacy on triple negative breast cancer
Year: 2025
Type: article
Abstract: A pH and GSH concentration dual responsive material was designed to address the insufficient efficacy and toxicity in triple negative breast cancer (TNBC) clinical chemotherapy. Methyl polyethylene glycol (mPEG) and oligomeric HA (o-HA) were conjugated to PLGA via hydrazone and disulfide bonds, respectively. The Adriamycin (ADR) and Olaparib (Ola) co-loaded dual responsive nano drug delivery system was prepared by double-emulsion method. The particle size was 173.78 ± 15.1 nm with a zeta potential of −9.88 ± 0.33 mV. Transmission electron microscopy revealed spherical nanoparticle (NP) with a bilayered structure. The encapsulation efficiencies for Adriamycin and Olaparib were 99.99 % and 73.91 %, respectively. The Drug loading was 4.76 % and 3.56 % accordingly. The particle size, potential and drug release profile demonstrated pH and GSH concentration sensitivity. The blank NP inhibited TNBC cells migration and invasion while showed good compatibility with L929 cell. The co-loaded NP showed exhibited synergistic cytotoxicity on TNBC cells and promoted apoptosis. The NP exhibited tumor targeting properties both in vitro and in vivo. Co-loaded NP showed significant stronger therapeutic effects in mice TNBC orthotopic tumor and lung metastasis models than the free drugs and the single-loaded NP with lower systemic toxicity.
Source: Materials & Design
Authors: Maidong Li, Xiaofeng Wan, Benjie Shan, Yang He, Chuanrong Chen, Ming Shen
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,650
OpenAlex ID: W4411442885