Work

Establishment and Application of Loop-Mediated Isothermal Amplification Assays for Pathogens of Rice Bakanae Disease

Year: 2025

Type: article

Abstract: Rice bakanae disease (RBD), a major threat in rice-cropping nations, can reduce rice yield and quality. As it is a seed-borne disease, effective seed detection is crucial. Loop-mediated isothermal amplification (LAMP) can rapidly and specifically amplify DNA at a constant temperature with high sensitivity. This research uses LAMP to develop rapid RBD pathogen detection systems. Primers were designed targeting the NRPS31 gene of Fusarium fujikuroi and conserved TEF1α sequences of Fusarium asiaticum, Fusarium proliferatum, and Fusarium andiyazi. These reactions at 60 °C for 60 min had a detection limit of 100 pg·μL−1, and LAMP proved applicable in field trials.

Source: Agriculture

DOI: 10.3390/agriculture15121319