Work

Protocol for establishing genetically engineered murine lung organoids mimicking cell plasticity and regeneration

Year: 2025

Type: article

Abstract: Lung endothelial cells (LuECs) play crucial roles in lung regeneration and support lung organoids (LOs). However, detailed guidelines for LO culture using LuECs have not been reported yet. Here, we present an efficient protocol for generating and culturing murine LOs that mimic regeneration. We describe steps for the isolation of LuECs and lung epithelial cells, followed by LO culture. We also describe how to establish genetically engineered LOs using viral transduction. For complete details on the use and execution of this protocol, please refer to Kim et al. 1 , 2

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103911

Authors: Bongjun Kim, Jae-Il Park

Institution: The University of Texas MD Anderson Cancer Center

Language: English

Topic: Tissue Engineering and Regenerative Medicine

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550