Year: 2025
Type: article
Abstract: Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for Phyllobates terribilis (12.6Gb), generated using a combination of sequencing platforms and bioinformatic approaches. Similar to other poison frog sequencing efforts, we recovered a highly fragmented assembly, likely due to the genome’s large size and very high repetitive content, which we estimate to be ≈ 88%. Despite the assembly’s low contiguity, we were able to annotate multiple members of three gene sets of interest (voltage-gated sodium channels and Notch and Wnt signaling pathways), demonstrating the usefulness of our assembly to the amphibian research community.
Source: Gigabyte
Authors: Roberto Marquez, Denis Jacob Machado, Reyhaneh Nouri, Kerry Gendreau, Daniel Janies, Ralph Saporito, Marcus Kronforst, Taran Grant
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $350
Awards: G1534155283, A multi-disciplinary approach to the study of amphibian diversification, A multi-disciplinary approach to the study of amphibian diversification: phase 2, G4402799499, G4982689826, G601850416, EDGE: Developing techniques for linking genotype to phenotype in amphibians, DISSERTATION RESEARCH: The evolutionary origin and genetics of color pattern diversity in Phyllobates poison frogs, Characterizing the Molecular Basis of Supergene Mimicry in Butterflies, G918765358
OpenAlex ID: W4411494837