Work

Isolation of Mitochondria From Yeast to Estimate Mitochondrial Pools of Inorganic Phosphate

Year: 2025

Type: article

Abstract: for quantitative estimation of inorganic phosphate or other metabolites. • Mitochondria are efficiently isolated by immunoprecipitation using magnetic beads, bypassing the need for time-consuming density-based centrifugation. • This method can be integrated into LC-MS-based workflows to quantify mitochondrial metabolites and proteins.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5370

Authors: Swagata Adhikary, Vineeth Vengayil, Sunil Laxman

Institutions: Manipal Academy of Higher Education, Institute for Stem Cell Biology and Regenerative Medicine

Language: English

Topic: Mitochondrial Function and Pathology

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W4411614349

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