Figure 2 from Histone Deacetylase Inhibitors Target DNA Replication Regulators and Replication Stress in Ewing Sarcoma Cells
Year: 2025
Type: other
Abstract: HDAC inhibitors downregulate the expression of genes related to DNA replication and the cellular response to DNA replication stress. A, EW8 cells were treated with panobinostat or romidepsin for 24 hours, and then cellular lysates and mRNA were collected for immunoblotting and RNA-seq analysis. B and C, Volcano plots of differentially expressed genes (fold change >2, adjusted P value < 0.05) in the EW8 cells treated with romidepsin (5 nmol/L; B) or panobinostat (10 nmol/L; C). D–E, RNA-seq data, fold change and adjusted P value, for the RRM1, RRM2, CHK1, and WEE1 genes in EW8 cells treated with romidepsin (5 nmol/L) and panobinostat (10 nmol/L). F and G, Gene sets (Kyoto Encyclopedia of Genes and Genomes pathway) enriched in the genes that are downregulated in EW8 cells treated with romidepsin (F) and panobinostat (G). H, RNA-seq data, fold change and adjusted P value, for the MCM2–7 genes in EW8 cells treated with romidepsin and panobinostat. I, EW8 and TC71 cells were treated with romidepsin (5 nmol/L) for 24 hours, and then cellular lysates were collected for immunoblotting.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4411705062