Figure 5 from Histone Deacetylase Inhibitors Target DNA Replication Regulators and Replication Stress in Ewing Sarcoma Cells
Year: 2025
Type: other
Abstract: HDAC inhibitors decrease BRD4 protein levels in Ewing sarcoma cells. A and B, Comparison of protein expression, based on RPPAs, in EW8 (A) or TC71 (B) cells treated with romidepsin (5 nmol/L) or panobinostat (10 nmol/L) for 24 hours. C, Overlap of proteins downregulated >1.5-fold in the RPPA experiment by romidepsin and panobinostat in Ewing sarcoma (ES6, EW8, and TC71) and osteosarcoma (U2OS) cell lines. D, Relative BRD4 mRNA in EW8 and TC71 cells treated with romidepsin (5 nmol/L). E, EW8 and TC71 cells were treated with romidepsin or panobinostat for 24 hours, and then cellular lysates were collected for immunoblotting for BRD4. F, CRISPR and shRNA dependency scores for BRD4 (Dependency Map, Broad Institute) in Ewing sarcoma and other cancer cell lines. G, EW8 and TC71 cells were treated with dBET1 (5 μmol/L) for 18 hours, and then cellular lysates were collected for immunoblotting for BRD4. H, Representative cell-cycle analysis (EdU and propidium iodide) plot for EW8 cells treated with dBET1 (5 µmol/L) for 18 hours. I, Summary of EdU incorporation into DNA for additional Ewing sarcoma cell lines treated with dBET1 (5 µmol/L) for 18 hours. P values were calculated using a two-tailed Student t test. **, P < 0.01. FC, fold change.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4411705089