Figure 6 from Histone Deacetylase Inhibitors Target DNA Replication Regulators and Replication Stress in Ewing Sarcoma Cells
Year: 2025
Type: other
Abstract: HDAC inhibitors downregulate survivin (BIRC5) in Ewing sarcoma cells. A, Treatment with romidepsin (5 nmol/L) causes morphologic changes in Ewing sarcoma cells. B, EW8 and TC71 cells were treated with romidepsin for 24 or 30 hours, and then cellular lysates were collected for immunoblotting. C, RNA-seq data, fold change and adjusted P value, for the survivin gene in EW8 cells treated with romidepsin and panobinostat. D, Ewing sarcoma cell lines were treated with romidepsin (5 nmol/L) for 24 hours, and then cellular lysates were collected for immunoblotting for survivin. E, Survivin mRNA expression level and CRISPR dependency score in Ewing sarcoma, other sarcoma, and other cancer cell lines. F, EW8 and TC71 cells were treated with YM155 for 24 hours, and then cellular lysates were collected for immunoblotting for survivin. G, EW8 cells were treated with different doses of YM115 for 72 hours, and then cell viability was measured using the alamarBlue assay. Error bars represent the mean ± SD of three technical replicates. The results are representative of two independent experiments. H, Kaplan–Meier analysis shows the overall survival of patients with Ewing sarcoma according to the expression level of survivin mRNA. P values were calculated using a one-way ANOVA followed by the Dunnett multiple comparisons test. TPM, transcripts per million. ***, P < 0.001.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4411705113