Trichophyton rubrum inhibits Candida albicans filamentation and its gene expression when grown in biofilms in vitro
Year: 2025
Type: article
Abstract: BACKGROUND: Dermatomycoses are caused by various fungi, including dermatophytes and Candida species, which are the most prevalent in isolated or associated forms. A great number of virulence factors expressed by these fungi are important for infection, and biofilm formation leads to the persistence of these infections. OBJECTIVES: This work aimed to evaluate the dynamics of Candida albicans filamentation genes in biofilms formed by Candida albicans and Trichophyton rubrum. METHODS: The effect of the supernatants on the biofilms was assessed by XTT reduction assay, confocal microscopy, and gene expression profile analysis by real-time polymerase chain reaction (RT-PCR). FINDINGS: The supernatants did not reduce the metabolic activities or damage the topography of the monospecies biofilms but caused a reduction in their thickness. The filamentation of C. albicans was inhibited when both fungi were cultivated directly. The filamentation genes studied (CPH1, HWP1, and EFG1) were negatively modulated in C. albicans. MAIN CONCLUSIONS: Our findings suggest that the antagonistic relationship shown by T. rubrum against C. albicans may be attributed to alterations of C. albicans filamentous genes.
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Funders: McKnight Foundation, Fundação de Amparo à Pesquisa do Estado de São Paulo, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Universidade Estadual Paulista, National Institutes of Health, National Institute of Allergy and Infectious Diseases
Awards: G1099234986, G1393966970, G1571466545, G1700182949, G1783826397, G2189176138, G2480813217, G2506626971, G2796550990, G2818788825, G2823776494, G3437701673, G3757914722, G3914732244, G4018344908, G4483470178, G47252533, G4941004437, G5162144583, G534316467, G5431258104, G5612830569, Dermatophytes and Dermatophytosis: biofilm formation and development of control strategies., G6912768488, G6965385692, G7596868111, G7785832706, G7881792348, G809170560, G8245666178, G8406829733, G8791428355
OpenAlex ID: W4411737305