Work

A hand-operated microfluidic sample preparation-to-analysis workflow for simplifying the basophil activation test

Year: 2025

Type: article

Abstract: values. Activation levels of basophils from anonymous and presumed non-allergic donors in samples stored in the lyse/fix buffer for up to 7 days at 4 °C are similar to those analyzed on day 0. In summary, we demonstrate the potential of μF-prep to facilitate access to the BAT by simplifying sample preparation, stabilizing samples to remove the need for overnight blood shipping for flow cytometry analysis, and automating the data analysis pipeline.

Source: Lab on a Chip

DOI: 10.1039/d5lc00037h

Authors: Nicolas Castaño, Kaiser Chua, An Nguyen, Wyeth Marshall, Grady H. Hofmann, Jonathan Lee, Mindy Tsai, Sayantani B. Sindher, Kari C. Nadeau, R. Sharon Chinthrajah, Stephen J. Galli, Sindy K. Y. Tang

Institutions: Stanford University, Harvard University

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Wallace H. Coulter Foundation, Stanford Byers Center for Biodesign, Stanford SystemX Alliance, Stanford Bio-X, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering

Awards: G1560375249, An Integrated Micro-Basophil Activation Test for Rapid Food Allergy Diagnostics, G5552045317, G604362868, Integrated Genomic and Functional Studies of Immunotherapy for Multi-Food Allergy

OpenAlex ID: W4411742899

FWCI: 0.94

Cites: 64

Cited by: 4

Related to: 10